Applied Biosystems 7500 - Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System

STANDARD
STANDARD
STANDARD
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 21
Notes
Chapter 4
4
Generating Data from RQ Plates –
7300 or Standard 7500 System
Prepare the
PCR Master Mix
Create a new
RQ Plate document
Prepare the
reaction plate
Save the
RQ Plate document
View RQ plate data
Program the
thermal cycling conditions
Start the run
OR
Generating
Data from
RQ Plates
Standard
STANDARD
Performin
g
a
n R
Q
S
tud
y
D
es
i
gn
i
ng
a
n R
Q
E
xper
i
ment
Performing
R
everse
Transcriptio
n
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
R
everse
P
rime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3
´
5
´
c
DNA
I
ntr
oduc
t
ion
a
nd Exam
p
le
R
Q
Experiment
G
eneratin
g
D
a
t
a
fr
o
m
R
Q
Plates
F
ast
Seepage25
Seepage30
Seepage32
Seepage32
Seepage22
Seepage23
See page 33