Applied Biosystems 7500 Getting Started Guide

STANDARD
STANDARD
STANDARD
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 21
Notes
Chapter 4
4
Generating Data from RQ Plates –
7300 or Standard 7500 System
Prepare the
PCR Master Mix
Create a new
RQ Plate document
Prepare the
reaction plate
Save the
RQ Plate document
View RQ plate data
Program the
thermal cycling conditions
Start the run
OR
Generating
Data from
RQ Plates
Standard
STANDARD
Performin
g
a
n R
Q
S
tud
y
D
es
i
gn
i
ng
a
n R
Q
E
xper
i
ment
Performing
R
everse
Transcriptio
n
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
R
everse
P
rime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3
´
5
´
c
DNA
I
ntr
oduc
t
ion
a
nd Exam
p
le
R
Q
Experiment
G
eneratin
g
D
a
t
a
fr
o
m
R
Q
Plates
F
ast
Seepage25
Seepage30
Seepage32
Seepage32
Seepage22
Seepage23
See page 33

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)