Applied Biosystems 7500 Getting Started Guide

FAST
FAST
FAST
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 37
Notes
Chapter 5
5
Generating Data from RQ Plates –
7500 Fast System
Prepare the
PCR Master Mix
Create a new
RQ Plate document
Prepare the
reaction plate
Save the
RQ Plate document
View RQ plate data
Program the
thermal cycling conditions
Start the run
OR
Generating
Data from
RQ Plates
Fast
FAST
Performin
g
an R
Q
S
tud
y
D
es
i
gn
i
ng
an R
Q
E
xper
i
men
t
Performin
g
R
everse
Transcriptio
n
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
Revers
e
Prime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
O
ligo d(T) or random hexame
r
3
´
5
´
cDNA
I
ntr
oduc
t
ion
and Exam
p
l
e
R
Q
Experiment
G
eneratin
g
D
a
t
a
fr
o
m
R
Q
Plates
S
tandard
STANDARD
TANDAR
Seepage43
Seepage48
Seepage51
Seepage51
Seepage38
Seepage40
See page 52

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)