Applied Biosystems 7500 Getting Started Guide

Chapter 6 Analyzing Data in an RQ Study
Configuring Analysis Settings
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 63
Notes
6
Configuring Analysis Settings
After you create the RQ Study document, you must
specify parameter values for the analysis.
Unless you have already determined the optimal
baseline and threshold settings for your experiment,
use the automatic baseline and threshold feature of the
SDS Software 1.3.1 (auto Ct), explained below. If the
baseline and threshold were called correctly for each
well, you can proceed to view the results. Otherwise,
you must manually set the baseline and threshold as
explained in “Manual Baseline and Threshold
Determination” on page 65.
To configure analysis settings:
1. Click or select Analysis > Analysis Settings.
2. In the Detector drop-down list, select All.
3. Select Auto Ct. The SDS Software 1.3.1
automatically generates baseline and threshold
values for each well.
IMPORTANT! After analysis, you must verify
that the baseline and threshold were called
correctly for each well, as explained in
“Adjusting the Baseline and Threshold” on
page 65.
Alternatively, you can select Manual Ct and
specify the threshold and baseline manually.
4. Select the Calibrator Sample.
Note: If your experiment uses only a single
plate, there must be at least two different samples
that have different names and have their own
endogenous controls. (You can go back to a
saved RQ Plate document and change the sample
names, if necessary.)
5. Select the Endogenous Control Detector.
2
3
4
5
6
7
8

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)