Appendix A
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 79
Notes
A
Creating Detectors
Before you can use a plate document to run a plate,
you need to create and apply detectors for all samples
on the plate. A detector is a virtual representation of a
gene- or allele-specific nucleic acid probe reagent
used for analyses performed on instruments.
To create a detector:
1. Select Tools > Detector Manager.
Note: A plate document (any type) must be open
before you can access the Tools menu.
2. Select File > New.
3. In the New Detector dialog box, enter a name for
the detector.
IMPORTANT! The name of the detector must be
unique and should reflect the target locus of the
assay (such as GAPDH or RNase P). Do not use
the same name for multiple detectors.
4. Optionally, click the Description field, then
enter a brief description of the detector.
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