Applied Biosystems 7500 Getting Started Guide

Appendix A
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 79
Notes
A
Creating Detectors
Before you can use a plate document to run a plate,
you need to create and apply detectors for all samples
on the plate. A detector is a virtual representation of a
gene- or allele-specific nucleic acid probe reagent
used for analyses performed on instruments.
To create a detector:
1. Select Tools > Detector Manager.
Note: A plate document (any type) must be open
before you can access the Tools menu.
2. Select File > New.
3. In the New Detector dialog box, enter a name for
the detector.
IMPORTANT! The name of the detector must be
unique and should reflect the target locus of the
assay (such as GAPDH or RNase P). Do not use
the same name for multiple detectors.
4. Optionally, click the Description field, then
enter a brief description of the detector.
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Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)