AccuPrime
™
Taq High Fidelity Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 25 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 94.0 94.0 4.0
Hold Time 15 15 Infinite
Step 2 RampRate — 6.0 — —
Temperature 52.0
Hold Time 15
Step 3 RampRate — 6.0 — —
Temperature 68.0
Hold Time 60
AmpliTaq
™
360 Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 94.0 95.0 72.0
Hold Time 180 95.0, 30 420
Step 2 RampRate — 6.0 4.0,
Infinite
—
Temperature 58.0
Hold Time 30
Step 3 RampRate — 6.0 — —
Temperature 72.0
Hold Time 60
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
70
SimpliAmp
™
Thermal Cycler User Guide
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