Applied Biosystems SimpliAmp Thermal Cycler - Page 71

AmpliTaq Gold
™
360 Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 95.0 95.0 72.0
Hold Time 300 15 420
Step 2 RampRate — 6.0 6.0 —
Temperature 55.0 4.0
Hold Time 30 Infinite
Step 3 RampRate — 6.0 — —
Temperature 72.0
Hold Time 60
AmpliTaq Gold
™
Fast PCR Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 95.0 96.0 72.0
Hold Time 600 3 10
Step 2 RampRate — 6.0 6.0 —
Temperature 62.0 4.0
Hold Time 3 Infinite
Step 3 RampRate — 6.0 — —
Temperature 68.0
Hold Time 5
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
SimpliAmp
™
Thermal Cycler User Guide 71