Applied Biosystems SimpliAmp Thermal Cycler - Page 76

Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Step 2 RampRate 50 105.0— 6.0 — —
Temperature 55.0
Hold Time 30
Step 3 RampRate — 6.0 — —
Temperature 68.0
Hold Time 60
General PCR Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 95.0 95.0 72.0
Hold Time 60 15 420
Step 2 RampRate — 6.0 6.0 —
Temperature 55.0 4.0
Hold Time 15 Infinite
Step 3 RampRate — 6.0 — —
Temperature 72.0
Hold Time 30
Platinium
®
PFX 2-Step Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 94.0 94.0 4.0
Hold Time 120 15 Infinite
Step 2 RampRate — 6.0 — —
Temperature 68.0
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
76 SimpliAmp
™
Thermal Cycler User Guide