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DeNovix DS-11 Series - Page 16

DeNovix DS-11 Series
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Basic Operation: Absorbance
Auto Run Measurements
1. Perform a Blank measurement as previously described and then select the
Auto Run checkbox on the app Run screen. Pipette 1 μL of the sample
solution onto the lower sample surface.
2. Lower the arm for automatic measurements. Deselect the feature to perform
new Blank measurements. Note: This feature may be used for both
microvolume and cuvette mode measurements.
Sample Measurement Modes
Measurement mode selections are made using the drop-down menu found
next to the Measure button on the Run screen.
The Microvolume mode uses 1.0 μL samples pipetted onto the sample
surface.
The microvolume Short Path mode requires 0.5 μL samples pipetted onto the
sample surface. This mode is for high concentration samples and does not use
the 0.5 mm pathlength.
The microvolume Fast Mode option is primarily for samples in the range
measured by the 0.5 mm pathlength although it may be used for higher
concentration samples .
The DS-11+ enables the use of 10, 5, 2, 1, 0.5, 0.2 and 0.125 mm pathlength
Cuvette modes.
Report screens will include actual absorbance values measured using a
specific cuvette pathlength. Graph screens will display all cuvette
absorbance values normalized to a 10 mm value.
A new Blank measurement must be made when changing between
microvolume and cuvette modes.
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