Best Practices
•
Ensure all sample concentrations in the thin walled assay tubes fall within the
limits of the assay or reagent kit for accurate results.
•
Use 200 μL volumes in a clear thin walled PCR tube.
•
Lower the cover before tapping the measure button.
•
Avoid introducing air bubbles into the sample solution when mixing samples.
•
Follow assay reagent manufacturers' recommendations regarding
temperature, incubation time, and protection from ambient light.
•
Follow assay reagent manufacturers' recommendations regarding suggested
standard curve concentrations and data analysis.
•
Ensure all samples and standards are treated identically in terms of incubation
times and temperature.
•
Minimize assay tube and solution temperature fluctuations as these may
impact the accuracy of the assay. Samples at higher temperatures will
generally have lower fluorescence.
•
Do not label the side of an assay tube as this could interfere with the sample
measurement.
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