Best Practices Absorbance Measurements
•
Clean both sample measurement surfaces prior to making the Blank
measurement.
•
Use dH
2
O, not detergents or alcohol, for routine sample measurement surface
cleaning.
•
Use a dry lab wipe to remove measurement liquids from both the top and
bottom surfaces immediately after each measurement.
•
Use 1 μL samples for routine microvolume measurements.
Note: Select the Short Path mode for high concentration 0.5 μL samples.
•
Use a fresh aliquot for each measurement.
•
Use a fresh tip to deliver each sample aliquot.
•
Avoid introducing bubbles when pipetting samples onto the measurement
surfaces.
•
Ensure sample concentrations fall within absorbance limits of the instrument
for accurate results.
•
Use the same buffer a sample is suspended in for the Blank measurements.
•
Avoid using buffers such as RIPA buffers that contain components with strong
absorbances at the wavelength of interest.
•
Measure a fresh aliquot of the buffer using the Measure button and ensure the
spectrum is relatively flat along the baseline before measuring samples.
•
For cuvette mode measurements, use cuvettes with 8.5 mm Z heights. Select
the Cuvette option using the drop-down menu next to the Measure button and
ensure the cuvette is inserted in the proper orientation.$
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