5. Absorbance Apps: Basic Operation
Microvolume Measurements
1. Ensure both top and bottom sample surfaces are clean.
2. Pipette 1 μL of the blank solution onto the lower sample surface. Lower the
top arm and tap the Blank button.
3. Remove the solution from both sample surfaces using a clean, dry lab wipe.
4. Pipette 1 μL of the sample solution onto the lower sample surface.
5. Lower the arm and tap the Measure button. All microvolume measurements
are reported in 10 mm equivalent absorbance values.
Cuvette Measurements
Refer to the light path arrow as a guide when inserting a cuvette. Use cuvettes
that meet the following specifications:
• Width: 12.5 mm Length: 12.5 mm Height: 45 mm Z height: 8.5 mm
• UV wavelength region: Use quartz or UV transparent plastic cuvettes
• Visible wavelength region: Use quartz or plastic cuvettes
1. Insert a cuvette pre-filled with the Blank solution. Lower the arm and tap the
Blank button.
2. Insert a cuvette pre-filled with the sample solution. Lower the arm and tap the
Measure button.
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