10. Absorbance OD 600 App
This application is generally used to determine the optical density of microbial
cell cultures at 600 nm. Conversions to cells per mL are based upon a user-
defined factor.
Quick Protocol
1. Launch the app from the Home screen.
2. Establish a Blank using the appropriate buffer.
3. Enter a sample name.
4. Enter a cell conversion factor (optional).
5. Measure a fresh aliquot of sample using the Measure button.
Notes:
•All cell type specific conversion factors are user-derived.
•Ensure culture is well mixed prior to sampling.
•Use fresh 1 uL aliquots for each microvolume measurement.
•Although microvolume OD 600 measurements can be made, the cuvette 10 mm mode
is recommended for this app. This option is available on the DS-11 FX +.!
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