DeNovix DS-11 Series User Manual

10. Absorbance OD 600 App
This application is generally used to determine the optical density of microbial
cell cultures at 600 nm. Conversions to cells per mL are based upon a user-
defined factor.
Quick Protocol
1. Launch the app from the Home screen.
2. Establish a Blank using the appropriate buffer.
3. Enter a sample name.
4. Enter a cell conversion factor (optional).
5. Measure a fresh aliquot of sample using the Measure button.
Notes:
•All cell type specific conversion factors are user-derived.
•Ensure culture is well mixed prior to sampling.
•Use fresh 1 uL aliquots for each microvolume measurement.
•Although microvolume OD 600 measurements can be made, the cuvette 10 mm mode
is recommended for this app. This option is available on the DS-11 FX +.!
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OD 600
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DeNovix DS-11 Series Specifications

General

Wavelength Range	190 - 840 nm
Minimum Sample Size	0.5 µL
Sample Volume	0.5 - 2 µL
Absorbance Precision	0.002 AU (1 cm equivalent)
Operating System	Android
Display	7-inch touchscreen
Power Requirements	100 - 240 V, 50/60 Hz
Pathlength	0.5 mm (auto-ranging)