1. Introduction 5 ..............................................................................................
2. Set up and Safety 6 .....................................................................................
3. Software Quick Guides 8 ............................................................................
Software Icons 8 .......................................................................................
Software Buttons 9 .....................................................................................
Software Navigation 10 ..............................................................................
Top Bar Overflow Functions 11 ...................................................................
4. Report and Graph Screens 12 ....................................................................
5. Absorbance Apps: Basic Operation 15 ....................................................
Typical Absorbance Run Screen 17 ...........................................................
Best Practices Absorbance Measurements 19 ...........................................
6. Absorbance Nucleic Acid Apps 20 ............................................................
dsDNA, RNA and ssDNA 21 ......................................................................
Microarray 22 ..............................................................................................
7. Absorbance Purified Protein, Peptide Apps 28 ........................................
Protein A280 29 ..........................................................................................
Labeled Proteins 31 ....................................................................................
Peptides 33 ................................................................................................
8. Absorbance Colorimetrics App 36 ............................................................
Generating a Standard Curve 37 ...............................................................
BCA 40
Bradford 40
Lowry 40
Pierce 660 40 .............................................................................................
9. Absorbance UV-Vis App 41 ........................................................................
10. Absorbance OD 600 App 43 ......................................................................