21. Troubleshooting
Quick Help
Most issues concerning accuracy, reproducibility, negative spectra and low
nucleic acid purity ratios are sample or technique related and are resolved by
following the suggestions below:
•
Establish a new Blank using the appropriate buffer.
•
Ensure that the sample isolation procedure is optimized and that samples are
purified when required prior to making absorbance measurements.
•
Ensure all solutions are homogenous and well-mixed prior to sampling.
•
Ensure sample concentrations fall within the absorbance limits of the
instrument. In the case of fluorescence assays, ensure sample concentrations
fall within the reagent limits as described by the assay manufacturer.
Microvolume Mode
•
Ensure both top and bottom microvolume measurement surfaces are clean
prior to making the Blank measurement.
•
Always remove sample solutions from both the top and bottom measurement
surfaces using dry, lint-free wipe immediately after each measurement is
complete.
•
Use calibrated pipettors and properly fitting tips to ensure a full 1 μL aliquot is
delivered to the sample measurement surface. Protein samples sometimes
wick up on the outside of the tip and may not be properly dispensed.
•
Use a clean pipette tip and a new aliquot of sample for each microvolume
measurement. $
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