Cuvette Mode
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Use cuvettes with Z heights of 8.5 mm.
•
Use quartz or UV transparent cuvettes for UV range measurements.
•
Ensure cuvettes are clean prior to use.
•
Fill cuvettes to manufacturer's specifications.
•
Confirm the mode selection has been changed on the Run screen to the
proper cuvette pathlength prior to a measurement.
•
Ensure cuvettes are properly inserted following the etched arrow light path.
•
Refer to a reagent kit manufacturer for troubleshooting colorimetric assays.
Fluorescence Mode
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Use 200 μL volumes in a clear thin walled PCR tube
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Avoid introducing air bubbles into the sample solution when mixing samples.
•
Ensure all sample concentrations in the thin walled assay tubes fall within the
limits of the assay or reagent kit for accurate results.
•
Follow assay reagent manufacturers' recommendations regarding
temperature, incubation time, and protection from ambient light.
•
Follow assay reagent manufacturers' recommendations regarding suggested
standard curve concentrations and data analysis.
•
Confirm that the correct LED excitation source was used for all user-defined
assays and methods.
•
Confirm that standard concentrations and dilutions were performed correctly.
•
Confirm that the correct concentration units for the standard curve and the
unknown samples were used to calculate the stock concentrations.
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