dsDNA, RNA and ssDNA
The basic nucleic acid apps are optimized for the factor used to calculate sample
concentrations.
Screen Features
•
Sample Type: The sample type is used to determine which ng-cm/μL factor is
used to calculate the concentration.
• The sample type is fixed for both the dsDNA and RNA apps.
• A Factor Input option along with the default ssDNA sample type is
available from the selector field for the ssDNA app.
•
Factor: The default wavelength-dependent factors used for each app are as
follows: dsDNA: 50 ng-cm/μL RNA: 40 ng-cm/μL ssDNA: 33 ng-cm/μL
• Oligo factors derived from the Calculator app may be copied and pasted
directly into this field.
•
Concentration Results: Concentration results are reported in units of ng/μL.
• Units may be changed using the Run screen top action bar Overflow
menu. The units selected at the time of the measurement is what is saved
to the database.
•
A260: Reports the baseline-corrected 260 nm absorbance value.
•
260/230: Displays the ratio of the 260 nm and 230 nm absorbance values.
•
260/280: Displays the ratio of the 260 nm and 280 nm absorbance values.
Note: The DNA and RNA apps do not distinguish between the nucleic acid
species within a sample. It is important to optimize the isolation protocol to
ensure accurate quantitation.$
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