6. Absorbance Nucleic Acid Apps
The four preconfigured nucleic acid apps are used to quantify nucleic acid
samples based upon absorbance values at 260 nm.
Quick Protocol
1. Launch the appropriate application from the Home screen.
2. Establish a Blank using the appropriate buffer.
3. Enter a sample name.
4. Select a sample type if applicable.
•
Enter optional user-defined ng-cm/μL factor (ssDNA and Microarray).
•
Select a dye type (Microarray only).
5. Measure a fresh aliquot of sample using the Measure button.
Notes:
•
Blank with TE if the sample is dissolved in TE.
•
Ensure that the sample isolation procedure is optimized and that samples are
purified prior to making absorbance measurements.
•
Ensure all solutions are homogenous and well-mixed prior to sampling.
•
Use fresh aliquots for each microvolume measurement.!
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