DeNovix DS-11 Series - Labeled Proteins

Purified Protein, Peptides: Absorbance
Labeled Proteins
The Labeled Protein application is used to quantify protein samples based upon
absorbance values at 280 nm as well as Dyes and Fluorophores at specific
analysis wavelengths.
Screen Features
•
Sample Type: The sample type and associated mass extinction coefficients
options include:
•
Dye Type: Cy3 is default selection for this application.
•
Protein Concentration Results: Results are reported in units of mg/mL.
• Units may be changed using the Run screen top action bar Overflow
menu. The units selected at the time of the measurement is what is saved
to the database.
•
Dye/Fluorophore Concentration Results: Results are reported in units of μM.
•
A280*: Reports the baseline and dye corrected 280 nm absorbance value.
This value may be lower than the A280 value observed in the absorbance
spectra due to the correction factors applied.
•
AXXX: The baseline-corrected dye specific analysis wavelength absorbance
value (e.g A550 when using the Cy3 dye).
Sample Type
E1% at 280 nm for 10 mm pathlength
BSA
6.67
1A=1mg/mL
10
IgG
13.7
E1%
User-defined mass extinction coefficient (L gm
-1
cm
-1
) for a 10
mg/mL (1%) solution
MW & Ext Coeff
User-defined MW (Daltons) and molar extinction coefficient
(M
-1
cm
-1
)
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