DeNovix DS-11 Series - Page 30

Purified Protein, Peptides: Absorbance
Baseline Correction
Bichromatic normalizations correct for baseline offsets due to sample
particulates. If a baseline correction is not used, the sample spectrum may be
offset from the baseline, resulting in a change in the reported sample
concentration. The DS-11 uses a correction at 340 nm as the default selection.
However, the user may elect to define the baseline nm used for specific
measurements.
. However, the user may elect to define the baseline nm used for specific
measurements.
The baseline correction screen is accessed from the Run screen top action bar
Overflow icon. Once a baseline correction nm is selected, it is applied to all
subsequent measurements within the same measurement session.
30
Sample Type
E1% at 280 nm for 10 mm pathlength
BSA
6.67
1A=1mg/mL
10
IgG
13.7
E1%
User-defined mass extinction coefficient (L gm
-1
cm
-1
) for a 10
mg/mL (1%) solution
MW & Ext Coeff
User-defined MW (Daltons) and molar extinction coefficient
(M
-1
cm
-1
)