Microarray
The Microarray app is used to quantify nucleic acid samples based upon
absorbance values at 260 nm and dyes based upon dye specific analysis
wavelengths.
Screen Features
•
Sample Type: The sample type selection of ssDNA is the default option for
this application. Options include:
• ssDNA (Factor = 33 for nucleic acid concentration calculation.)
• RNA (Factor = 40 for nucleic acid concentration calculation.)
• Factor Input to enable a user-defined nucleic acid factor. Oligo factors
derived from the Calculator app may be copied and pasted directly into
this field.
•
Dye Type: Cy3 is default selection for this application.
•
Nucleic Acid Concentration Results: Results are reported in units of ng/μL.
• Units may be changed using the Run screen top action bar Overflow
menu. The units selected at the time of the measurement is what is saved
to the database.
•
Dye/Fluorophore Concentration Results: Results are reported in units of μM.
•
A260*: Reports the baseline and dye corrected 260 nm absorbance value.
This value may be lower than the A260 value observed in the absorbance
spectra due to the correction factors applied.
•
AXXX: The baseline-corrected dye specific analysis wavelength absorbance
value (e.g A550 when using the Cy3 dye).$
To Next Page
Loading...