Microvolume Pathlength Verification
The DeNovix LC-NA verification fluid is supplied as single use vials composed of
a non-toxic solution of aqueous nicotinic acid. The solution is used to verify that
the microvolume pathlengths are within specifications.
Procedure
1. Ensure both sample measurement surfaces are clean. Refer to the general
cleaning instructions in the chapter entitled "Maintenance" for more
information.
2. Launch the Diagnostics app and navigate to the Verification tab.
3. Enter the lot specific Target Absorbance stated on the ampule in the
appropriate field.
4. Add 1 μL of dH
2
O to the pedestal, lower the arm and click Blank.
5. Wipe the upper and lower pedestals using a dry, lint-free laboratory wipe.
6. Vigorously shake the nicotinic acid ampule to thoroughly mix the solution.
Ensure all of the solution is in the bottom portion of the ampule before opening.
7. Pipette 1μL of the solution onto the sample surface, lower the arm and click
Measure.
8. Wipe the sample off both the top and bottom sample surfaces.
9. Repeat steps 7 and 8 for a total of 5 measurements using fresh aliquots for
each measurement.$