Preparations
T
he alarm for the air sensor located before the column must be disabled before filling
the system/column with liquid (Alarms →Air_Alarm →Disabled).
Connect an empty column
Follow the instruction below to connect an empty column to ÄKTA ready XL.
Step Action
1 Connect tubing between the system valve marked C
OLUMN BOTTOM
and the column bottom connection.
2 Connect tubing between the system valve marked COLUMN TOP and the
column top connection.
Connect a packed column without
b
ypass lines/valves
Follow the instruction below to connect a pre-packed column without bypass lines/
valves to ÄKTA ready XL.
Step Action
1 Connect tubing to the system valve marked C
OLUMN BOTTOM, but do
not connect the other end of the tubing to the bottom of the column at this
time.
2 Set the system column valves to the UpFlow position.
3 Using the pump, fill the system with an appropriate liquid for column in-
stallation.
4 When the system, including the tubing connected to the column bottom
system valve, is filled with liquid, connect the other end of the tubing to the
bottom of the column.
5 Connect tubing between the top of the column and the system valve
marked COLUMN TOP.
Note:
If the column will be run in downflow mode after the connection, the tubing
connected to the column top must also be filled with liquid before it is con-
nected to the column top.
6 Operation
6
.1 Connect a column
132
ÄKTA ready XL Operating Instructions 29281616 AE
*** TeDIS Valid on Date Printed Printed on: 15-09-2023 11:27:35 ***
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