Connect a packed column with
b
ypass lines/valves
Follow the instruction below to connect a pre-packed column with bypass lines/
valves to ÄKTA ready XL.
Step Action
1 Connect tubing between the column bottom and the system valve marked
C
OLUMN BOTTOM.
2 Connect tubing between the column top and the system valve marked
COLUMN TOP.
3 Set the system column valves to the UpFlow position.
4 Using the pump, fill the system with an appropriate liquid for column in-
stallation.
5 When the system, including the tubing connected to the column, is filled
with liquid, stop the pump.
6 Use the manual valves on the column to change from bypass to inline.
Final considerations
O
nce the column is connected, make sure to activate the system alarm or alarms
again after the filling sequence.
6 Operation
6
.1 Connect a column
ÄKTA ready XL Operating Instructions 29281616 AE 133
*** TeDIS Valid on Date Printed Printed on: 15-09-2023 11:27:35 ***
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