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Hettich ROTOFIX 32 A - Software Operation; Setting Centrifugation Parameters

Hettich ROTOFIX 32 A
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AB1206en_SARev.: 06 / 11.2023
28 / 39
Software operation
Display of the relative centri-
fugal force (RCF)
7 Software operation
7.1
Centrifugation parameters
7.1.1 Set the brake level
1. Switch off the mains switch.
2. Press and hold down the
[RPM/RCF x 100]
button and the
[IMPULSE]
button simultaneously.
3. Switch on the mains switch and release the buttons.
🡆 Press the
[RPM/RCF x 100]
button repeatedly until the speed
indicator shows the machine version and the time indicator shows
the brake level set (and/or
‘0’
or
‘1’
).
The machine version is set ex works and cannot be changed.
The machine version is set ex works and cannot be changed.
4. Use the
[t]
buttons to set the desired brake level.
🡆
Level 1 = short ramp-down time.
Level 0 = long ramp-down time.
5.
Press the
[STOP]
button to save the settings.
7.1.2
Relative centrifugal force, RCF
The relative centrifugal force RCF is dependent on the speed and the centri-
fuging radius.
The relative centrifugal force RCF is stated as a multiple of the acceleration
due to gravity (g).
The relative centrifugal force RCF is a dimensionless numerical value and is
used to compare the separation and sedimentation performance.
RCF = Relative Centrifugal Force
RPM = speed
r = centrifuging radius in mm = distance from the centre of the axis of
rotation to the bottom of the centrifuge tube.
1. Press and hold the
[RCF]
button during the centrifugation run.
🡆 Relative centrifugal force (RCF) is displayed.
2. Release the
[RCF]
button.
🡆 The speed is displayed.
7.1.3 Centrifugation of substances or mixtures of substances with a density higher than
1.2 kg/dm
3
The density of the substances or mixtures of substances must not exceed
1.2kg/dm³ during centrifugation at maximum speed. The speed must be
reduced for substances or substance mixtures with a higher density. The
permissible speed can be calculated using the following formula:

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