EMO Indicator
There are two EMO (Emergency Motion Off) switches; one on the front and one on the rear of the Echo Liquid Handler.
Gray: OK. The Emergency Motion Off switches are in their normal, not activated position.
Red: An Emergency Motion Off switch has been activated. Power has been turned off to many internal Echo electrical devices. For
a full discussion of the Emergency Motion Off switch, when it should be used, and how to power on an Echo Liquid Handler after
the Emergency Motion Off switch has been used, see Emergency Motion Off Switch.
Fluid Leak Indicator
Sensors inside the Echo Liquid Handler check for coupling fluid in the bubbler catch basin or lower pan.
Green: No fluid leak has been detected.
Red: Fluid has been detected in the lower pan or excess fluid has been detected in the catch basin. The coupling fluid pump has
been turned off. The user should call Labcyte Technical Support.
Compressed Air Pressure Indicator
A pressure sensor inside the Echo Liquid Handler monitors the user-supplied compressed air supply.
Green: OK. Air pressure is within specifications.
Red: The user-supplied compressed air is low <552 kPa (<80 psi) or nonexistent. Check for leaks, disconnections, or for the air
pressure turned off at the source.
Vacuum Supply Pressure Indicator
A pressure sensor monitors the user-supplied vacuum supply line pressure inside the Echo Liquid Handler.
Green: OK. Vacuum pressure is within specifications.
Red: The user-supplied vacuum pressure is low <78 kPa (<15 in Hg Vac) or nonexistent. Check for leaks, disconnections, or for the
vacuum turned off at the source.
Coupling Fluid Indicator
A sensor monitors the level of coupling fluid in its bottle.
Green: OK. Coupling fluid is above the minimum required level.
Yellow: The coupling fluid bottle level is low (<250 mL).
Note: When the coupling fluid falls below its recommended minimum level, bubbles may be introduced into the coupling
fluid, reducing the accuracy or precision of source plate surveys and liquid transfers. Labcyte recommends refilling the
coupling fluid bottle as soon as possible. For more information, see Echo Coupling Fluid Maintenance.
Waste Bottle Indicator
The waste bottle is not necessary for the Echo 525 Liquid Handler. The indicator will always be Green.
Focus Calibration Indicator
Periodically, the focal length of the acoustic transducer should be checked. When this period of time has expired, the Focus
Calibration indicator turns yellow. The user should perform a focus calibration as soon as possible. For more information, see
Focus Calibration Check.
Green. Focus calibration has been performed recently.
Yellow. A Focus calibration should be performed as soon as possible.
123 PN | 001-11665
USER GUIDE | Echo® 525 Liquid Handler User Guide Echo Liquid Handler Software
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