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Labcyte Echo 525 - Page 152

Labcyte Echo 525
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Clean and Refill Materials Required
The following materials are required to refill the coupling fluid bottle:
l Type 1 Ultrapure Water, sufficient to fill the 1 L bottle to ~900mL.
l Gloves for protection when handling the fluids
l Paper towels or shop cloths to deal with any unexpected fluid leaks
l An empty, clean 1000 mL coupling fluid bottle (square bottle catalog number ECHO-AL15-S)
The fluidic system will be emptied, purged, and refilled several times, therefore take note of the following caution:
Caution: When working with the Echo coupling fluid system and turning its coupling fluid pump on and off, make sure
that the vacuum line is connected and working before turning the coupling fluid pump back on. If the vacuum line is
disconnected or the vacuum system is not operating correctly, when the coupling fluid pump is turned on, the coupling
fluid can flood the bottom of the system.
Purge Echo Coupling Fluid
Caution: When turning the pump on and off for the Echo coupling fluid system, ensure the vacuum line is connected and
working before turning the pump back on. If the vacuum line is disconnected or the vacuum system is not operating
correctly when the pump is turned on, the coupling fluid can flood the bottom of the system.
Follow the steps below to purge the coupling fluid bottle:
1. Ensure that no fluid transfer or diagnostic procedures are under way.
2. Turn off the vacuum and coupling fluid pump.
l Turn off the coupling fluid pump through the Diagnostics tab of the Echo Liquid Handler software.
l Select the checkbox for Coupler Pump Off.
Note: The surge tank will add a few seconds to stopping the vacuum.
Maintenance Procedures CHAPTER 6 | Maintenance and Service
152

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