Molecular Devices SpectraMax M3 User Manual

SpectraMax M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
0112-0115 F 25
Figure 2-2 Excitation and emission spectra.
Because the intensity of the excitation light is usually many tens of
thousands of times greater than that of the emitted light, some type of
spectral separation is necessary to reduce the interference of the
excitation light with detection of the emitted light. The SpectraMax
Multi-Mode Microplate Reader incorporates many features designed to
restrict interference from reflected excitation light. Among these
features is a set of long-pass emission cutoff filters that can be set
automatically by the instrument or manually by the user. If the Stokes
shift is small, it may be advisable to choose an excitation wavelength
that is as far away from the emission maximum as possible while still
being capable of stimulating the fluorophore so that less of the excited
light overlaps the emission spectrum, allowing better selection and
quantitation of the emitted light.
Wavelength (nm)
Relative Fluorescence
Absorption
Excitation
maximum
Emission
maximum
Stokes
Shift
500 550 600 650
0
0.5
1.0

Molecular Devices SpectraMax M3 Specifications

General

Brand	Molecular Devices
Model	SpectraMax M3
Category	Laboratory Equipment
Language	English