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Molecular Devices SpectraMax M3 User Manual

Molecular Devices SpectraMax M3
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SpectraMax
®
M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
User Guide
0112-0115 F
July 2010

Table of Contents

Questions and Answers:

Molecular Devices SpectraMax M3 Specifications

General IconGeneral
TypeMulti-Mode Microplate Reader
Wavelength Range200 - 1000 nm
Light SourceXenon flash lamp
Wavelength SelectionMonochromator
SoftwareSoftMax Pro
Detection ModesAbsorbance, Fluorescence Intensity, Luminescence
Plate Formats6-, 12-, 24-, 48-, 96-, 384-well plates
Read SpeedLess than 10 seconds for a 96-well plate
ShakingLinear, Orbital
Power Requirements100-240 VAC, 50/60 Hz

Summary

SpectraMax Reader Overview

Computer Control with SoftMax Pro

Overview of instrument control via SoftMax Pro Software.

Control Panel Functions

Description of the LCD display and membrane keys for instrument control.

SpectraMax Principles of Operation

Absorbance Measurement Principles

Explanation of absorbance as light absorbed by a solution.

Optical Density and Transmittance

Definitions and formulas for optical density and transmittance.

PathCheck Pathlength Measurement Technology

How pathlength compensation normalizes absorbance values.

Water Constant vs. Cuvette Reference

Guidance on choosing between Water Constant and Cuvette Reference.

PathCheck Interfering Substances

Information on materials that may interfere with PathCheck measurements.

Time-resolved Fluorescence

Details on Time-resolved Fluorescence (TRF) measurements.

Fluorescence Polarization

Principles and calculations for Fluorescence Polarization (FP) mode.

SpectraMax Installation

Installation Safety and Cautions

Essential safety warnings and cautions before installing the instrument.

Setting up the Instrument Location

Guidelines for placing and connecting the instrument.

SpectraMax Operation

Optimizing Fluorescence Assays

Guidance on optimizing fluorescence assay settings.

Optimizing Absorbance Assays

Factors affecting absorbance assay optimization.

Excitation and Emission Wavelength Optimization

Procedure to optimize excitation and emission wavelengths for assays.

Spectral Scanning for Fluorescence Optimization

Using spectral scanning to determine optimal excitation/emission wavelengths.

Optimizing Time-resolved Fluorescence Assays

Settings and considerations for Time-resolved Fluorescence (TRF) assays.

Optimizing Fluorescence Polarization Assays

Best practices for optimizing Fluorescence Polarization (FP) assays.

Optimizing Luminescence Assays

Recommendations for optimizing luminescence assays.

SpectraMax Maintenance

Technical Support and Service

Information on contacting technical support for assistance.

General Maintenance Precautions

Important safety warnings and cautions for maintenance procedures.

SpectraMax Troubleshooting

Error Codes and Probable Causes

List of error code ranges and their likely causes.

Understanding Error Messages

Explanation of fatal and warning error messages.

SpectraMax Specifications

Performance Specifications Summary

Comprehensive technical specifications for the reader's performance.

SpectraMax Wavelengths

Common Wavelengths for Fluorescence and Luminescence

Tables listing excitation and emission wavelengths for various fluorophores.

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