2-1
Contents
1. System Configurations and Part Names............................................................................................2-3
1.1 Overview ........................................................................................................................................ 2-3
1.2 Optical Paths of Lasers .................................................................................................................. 2-5
1.2.1 Laser Unit........................................................................................................................... 2-5
1.2.2 Microscope Part (TIRF-PAU Illuminator and Ti-E System)................................................ 2-6
1.3 Safety Labels.................................................................................................................................. 2-7
1.3.1 Ti-U Microscope Body........................................................................................................ 2-8
1.3.2 Ti-E Microscope Body........................................................................................................ 2-9
1.3.3 LU4A Four-laser Module A (with LU4-B5 Beamsplitter 50/50 installed)............................. 2-10
1.3.4 LU-LR Four-laser PS Rack .............................................................................................. 2-11
1.3.5 TI-LU4SU Shutter Unit LU4 ............................................................................................. 2-12
1.3.6 TIRF-PAU Illuminator ....................................................................................................... 2-13
1.4 Name of Each Part ....................................................................................................................... 2-14
2. Preparations........................................................................................................................................ 2-25
2.1 Check Items and Tools................................................................................................................. 2-25
2.2 Overview of Setup Procedure ...................................................................................................... 2-26
3. Setting Up the PC and Operation Software ..................................................................................... 2-27
4. Setting Up the Microscope ................................................................................................................ 2-28
4.1 Overall Configuration ................................................................................................................... 2-28
4.2 Setup Procedure .......................................................................................................................... 2-29
4.2.1 Assembling Each Part and Attaching Them to the Microscope....................................... 2-29
4.2.2 Attaching the Laser Safety Kit.......................................................................................... 2-37
4.2.3 Attaching the Stage Up Kit ............................................................................................... 2-39
4.2.4 Attaching the Back Port Unit ............................................................................................ 2-42
5. Laser Unit ............................................................................................................................................ 2-44
5.1 Overview of the Laser Units ......................................................................................................... 2-45
5.1.1 LU4A Four-laser Module A (with LU4-B5 Beamsplitter 50/50 installed) .......................... 2-45
5.1.2 LU-LR Four-laser PS Rack .............................................................................................. 2-49
5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) ............................................. 2-51
5.2.1 Preparation....................................................................................................................... 2-51
6. Connection between the Laser Unit and the Microscope .............................................................. 2-56
6.1 Setting and Connecting the TI-LU4SU Shutter Unit LU4............................................................. 2-56
6.2 Attaching the Optical Fiber to the Light Source ........................................................................... 2-59
6.3 Replacement of Optical Path Switch Mirror ................................................................................. 2-61
6.4 Cable Connections (Four Laser Module A).................................................................................. 2-63
7. Confirmation and Check Sheet ......................................................................................................... 2-64
7.1 Checking Procedure..................................................................................................................... 2-64
7.2 Check Sheet................................................................................................................................. 2-65
8. Specifications and Performance....................................................................................................... 2-67
8.1 Overall Configuration ................................................................................................................... 2-67
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