Chapter 1 System Configurations and Part Names
1.2 Optical Paths of Lasers
2-6
1.2.2 Microscope Part (TIRF-PAU Illuminator and Ti-E System)
TIRF-PAU illuminator and Ti-E system (top view)
Figure 1.2-2
TIRF-PAU illuminator and Ti-E system (front left view)
C
A
U
T
IO
N
–
C
L
A
S
S
3
B
L
A
S
E
R
R
A
D
I
A
T
I
O
N
W
H
E
N
O
P
E
N
A
V
O
I
D
E
X
P
O
S
U
R
E
T
O
T
H
E
B
E
A
M
V
O
R
S
I
C
H
T
–
L
A
S
E
R
S
T
R
A
H
L
U
N
G
K
L
A
S
S
E
3
B
,
W
E
N
N
A
B
D
E
C
K
U
N
G
G
E
Ö
F
F
N
E
T
N
I
C
H
T
D
E
M
S
T
R
A
H
L
A
U
S
S
E
T
Z
E
N
A
V
O
ID
E
X
P
O
S
U
R
E
LASER
LIGHT
IS
EMITTED
FROM
THIS
APERTURE
C
A
U
T
I
O
N
–
C
L
A
S
S
3
B
L
A
S
E
R
R
A
D
I
A
T
I
O
N
W
H
E
N
O
P
E
N
A
V
O
I
D
E
X
P
O
S
U
R
E
T
O
T
H
E
B
E
A
M
V
O
R
S
I
C
H
T
–
L
A
S
E
R
S
T
R
A
H
L
U
N
G
K
L
A
S
S
E
3
B
,
W
E
N
N
A
B
D
E
C
K
U
N
G
G
E
Ö
F
F
N
E
T
N
I
C
H
T
D
E
M
S
T
R
A
H
L
A
U
S
S
E
T
Z
E
N
L
A
S
E
R
R
A
D
I
A
T
I
O
N
A
V
O
I
D
E
X
P
O
S
U
R
E
T
O
B
E
A
M
C
L
A
S
S
3
B
L
A
S
E
R
P
R
O
D
U
C
T
A
V
O
I
D
E
X
P
O
S
U
R
E
L
A
S
E
R
L
IG
H
T
IS
E
M
IT
T
E
D
F
R
O
M
O
B
J
E
C
T
I
V
E
A
P
E
R
T
U
R
E
Figure 1.2-3
The TV camera can be installed in a side port (right or left port) or the bottom port (only for Ti-E/B). To
change the optical path, use the optical path selector switch on the microscope.
Note that the laser shutter automatically closes when the microscope optical path is changed to the
eyepiece. It is not possible to observe images, obtained by irradiating the laser to the specimen, from the
binocular part of the microscope.
Laser light
Laser light
TIRF-PAU illuminato
Optical fibe
TV camera
Laser safety cover
Motorized epi-fl filter turret
Ti-E microscope body
Optical fibe
Laser light
(from the laser unit)
Laser light
(from the laser unit)