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PEAK INSTRUMENTS C-7200 Series - User Manual

PEAK INSTRUMENTS C-7200 Series
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User Manual PEAK INSTRUMENTS INC
C-7200 Series Spectrophotometer
User Manual and Maintenance Guide
PEAK INSTRUMENTS INC
Version 1801

Table of Contents

Questions and Answers

PEAK INSTRUMENTS C-7200 Series Specifications

General IconGeneral
ModelC-7200 Series
CategoryMeasuring Instruments
TypeDigital Multimeter
Continuity TestYes
Diode TestYes
Safety RatingCAT III 600V
DC Current Range0.1μA to 10A
AC Current Range600μA/6000μA/60mA/600mA/10A
Current Measurement Range0.1μA to 10A
Frequency Measurement Range0.1Hz to 10MHz

Summary

General Information

Safety Notes

Read the entire user manual carefully before you unpack, set up or operate the device.

Product Overview

Basic Operating Principles

Explains the nature of absorption, spectrophotometric analysis, and the Lambert Beer law.

Performance Indicators

Definitions

Defines key performance metrics like wavelength accuracy, repeatability, and spectral bandwidth.

Applications and Usage Conditions

Applications

Lists the various fields and industries where the spectrophotometer is used.

Using Conditions

Specifies environmental and installation requirements for optimal instrument operation.

Product Features and Installation

Features

Highlights key features like double-beam optical monochromator, LCD screen, and software capabilities.

Technical Specifications

Technical Data

Provides detailed specifications including model, wavelength range, accuracy, and dimensions.

Main Functions Overview

Function Diagram

Illustrates the instrument's main functions and their interrelationships.

Instrument Structure

Top View of Structure

Diagram showing the layout of components visible from the top of the instrument.

Bottom View of Structure

Diagram showing the layout of components visible from the bottom of the instrument.

Key Components and Light Path

Light Path Diagram

Illustrates the optical path of light through the instrument's components.

Important Parts of the Instrument

Details critical components like the Power Board and Light Room.

Instrument Installation

Installation Steps

Step-by-step guide for setting up the spectrophotometer in its operating environment.

Basic Operations and Controls

Panel Schematic Diagram

Visual layout of the instrument's control panel and buttons.

Button Function Descriptions

Explains the function of each button on the instrument's control panel.

Core Operations and Self-Test

Basic Operations

Describes fundamental operations like adjusting the blank and setting wavelength.

Instrument Self-test

Details the automatic self-checking process performed upon instrument startup.

Self-Test and Photometric Measurement

System Parameters Check

Covers system checks, baseline reading, and dark current calibration.

Photometric Measurement Function

Describes measuring absorbance, transmittance, or energy at a single wavelength.

Photometric Measurement Setup

Set Measuring Mode

Guides on selecting the measurement mode (Absorbance, Transmittance, Energy).

Set Wavelength

Instructions for setting the desired wavelength for photometric measurements.

Photometric Measurement Data Handling

Measurement Data

Details how sample data is recorded after calibration and testing.

Save and Delete Data

Procedures for saving test data to a file and deleting existing data.

Photometric Measurement File Operations

Open the File

Instructions on how to load previously saved photometric data files.

Quantitative Measurement

Build Standard Curve

Guide to creating a standard curve by inputting concentrations and measuring standard samples.

Quantitative Measurement Setup

Standard Sample Measuring

Procedure for measuring standard samples to build a calibration curve.

Quantitative Measurement Execution

Sample Test

How to test an unknown sample and obtain its concentration.

Open Standard Curve

Method for applying a previously created standard curve for sample analysis.

Time Scan (Kinetic) Analysis

Time Scan Function Description

Overview of the kinetic function for measuring trends over time.

Time Scanning Parameters

Setting parameters like time interval, test time, and measurement mode for kinetic scans.

Kinetic Scan Operations

Data Test and List

Performing the kinetic measurement and viewing the recorded data.

Kinetic Scan Data Analysis

Slope Calculation

Calculating the slope from kinetic data by defining start and end times.

Wavelength Scanning Function

Introduction to scanning absorbance/transmittance across a range of wavelengths.

Wavelength Scan Setup

Set Scanning Parameters

Configuring parameters for wavelength scanning like range, interval, and speed.

System Baseline

Procedure for creating a system baseline before performing wavelength scans.

Wavelength Scan Calibration

Create User Baseline (Corrected Blank)

Establishing a baseline using a reference sample (blank).

Begin Testing

Initiating the sample testing process after baseline setup.

Wavelength Scan Results

Peaks and Valleys Identification

Locating and identifying peaks and valleys in the scan results.

Data List

Viewing the detailed list of test data from the wavelength scan.

Multi-Wavelength Testing

Function Description

Explains measuring transmittance or absorbance at multiple wavelengths simultaneously.

Multi-Wavelength Setup

Set Parameters

Configuring the number of wavelengths and specific wavelengths for testing.

Correction 100%T;0 Abs

Performing blank correction for multi-wavelength measurements.

Multi-Wavelength Test Execution

Data Test

Performing the actual sample measurement in multi-wavelength mode.

Protein;DNA Measurement Function

Overview of dedicated tests for protein and nucleic acid concentration analysis.

Protein;DNA Measurement Setup

Parameter Selection

Choosing measurement modes and inputting coefficients for Protein/DNA tests.

Set Zero and Test

Steps for setting zero and performing the Protein/DNA measurement.

System Setup and Dark Current

System Setup Menu

Accessing the system setup menu for various instrument configurations.

Dark Current Measurements

Performing dark current measurements for instrument calibration.

System Setup: Lamps and Time

Deuterium Lamp Curve Calibration

Locating the deuterium lamp's characteristic curve for calibration.

Time and Date Settings

Configuring the instrument's internal clock and calendar.

System Setup: Light Sources

Light Source Management

Controlling and monitoring the status of deuterium and tungsten lamps.

System Setup: General and File Management

General Settings

Adjusting language, precision, beep, wallpaper, and font settings.

File System

Viewing file status, formatting disks, and deleting files.

System Setup: Storage and Restore

USB Storage Device Status

Checking the status of connected external USB flash drives.

Restore Factory Settings

Restoring the instrument to its default factory configuration.

System Information and Maintenance

System Information

Viewing software and hardware version details.

Cleaning Requirements

Guidelines and precautions for cleaning the instrument.

Cleaning Procedures

Spectrophotometer Cleaning

Instructions for cleaning the instrument housing, cell compartments, and accessories.

Display Cleaning

Proper methods for cleaning the instrument's display screen.

Cuvettes;Cells Cleaning

Steps for cleaning and rinsing sample cuvettes/cells.

Instrument Care and Lamp Maintenance

Routine Precautions

General precautions for maintaining instrument performance and longevity.

Tungsten Lamp Replacement

Step-by-step guide for safely replacing the tungsten lamp.

Lamp Replacement Procedures

Deuterium Lamp Replacement

Instructions for safely replacing the deuterium lamp.

Troubleshooting

Power Failure Issues

Common causes and resolutions for power-related problems with the instrument.

Troubleshooting Self-Test Failures

Self-Test Failures

Diagnosing and resolving issues detected during the instrument's self-test.

Troubleshooting Other Failures

Other Failures

Addresses less common issues like unstable energy or dirty optical parts.