Lambda 25, 35, 45 User’s Guide
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5. Block the sample and reference beam window on the right hand side of the
sample compartment with a card to prevent white light from saturating the
detector.
6. Return to standby display.
7. From the Application menu in UV WinLab select
Manual.
The lambda Manual Control dialog is displayed.
8. Set the
Wavelength to 0 nm and click Setup.
9. By holding a piece of matt white paper behind each cell holder, visually
examine the light spot to see that the radiation beam is passing through the cell
sample area.
10. Diffraction patterns become apparent if the radiation beam impinges on the cell
wall.
11. If the radiation beam is not centered exactly, loosen the two locking screws and
the two milled posts on the relevant cell holder and shift the cell holder plate to
center the radiation beam horizontally.
Then retighten the two milled posts and the two locking screws.
12. Now visually check the vertical alignment of the radiation beam in the cell
sample area.
Alignment is correct when the radiation beam is just above the floor of the cell
sample area (min. 2 mm) or covers the cell window.
NOTE: The center of the window for micro flowcells should be ideally approximately
15 mm above the base of the cell.
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