Photon Systems Instruments FMT150 - Page 25

© PSI (Photon Systems Instruments), spol. s r. o.
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lid also has three larger openings for optional sensors such as pH/Temperature, O
2
, CO
2
probes. If sensors are not used
these openings can be kept closed with the filler screws provided.
The stainless steel aeration tubing mounted to the bottom of the vessel lid is connected via two Luer Lock connectors with
the gas dispenser tubing (n°4 in Fig. 13A) linked via a T-piece to the humidifier bottle (Fig. 13B). The source gas for the
culture (gas pump or gas mixing system) is humidified before entering the culture vessel and this helps to maintain a
constant volume in the vessel. The third luer lock connector is linked with silicone tubing (n°7 in Fig. 13A,C), which serves
for removal of residual or overflow medium.
The longer metal thin tubing on the vessel lid is used for medium outlet.
The fourth luer outlet can be either closed by a Lock Ring Plug (component n°4 in Tab. 4) or connected with silicone tubing
and used as a sampling outlet or for medium in-flow when connected with peristaltic pump (n°6 in Fig. 13A,C).
Sampling tube can be used as gas outlet when Gas Analysis System (GAS) is connected to the PBR
FMT150; for more information see page 51.
Once the cultivation vessel is set up and placed in the photobioreactor it should be locked to the body of the PBR FMT150
by the clips on each side of the PBR.
Fig. 13 Components of the cultivation vessel 400 ml. A) vessel and the vessel lid; numbers correspond to description in C. B) Top of the
lid with connection of the aeration tubing. C) Vessel lid with one sensor and all tubing connections: (1a,b) Clips to lock the vessel to the
PBR. (2a,b) Holders for easy vessel manipulation. (3a,b) Screws for fastening part to the lid to the vessel. (4a,b) Inlets for the gas
dispenser tubing. (5) Connectors for optional sensors closed with filler screws. (6) Sampling tube. (7) Excess medium outlet. (8)
pH/temperature sensor.