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RoboSep™-S is an instrument that comprises four main mechanical components operated under computer
control (Figure 1):
Robotic Arm
Carousel
Tip Stripping Arm (not shown)
Hydraulic System (behind access panel)
With these systems acting in concert, RoboSep™-S performs the following actions to automate EasySep™
cell separation protocols:
Accurate dispensing of magnetic labeling reagents into cell samples using disposable 1 mL tips
Mixing and transfer of the sample between tubes using disposable 5 mL tips
Transfer of buffer solution from the buffer bottle to tubes on the carousel using disposable 5 mL tips
Simultaneous tracking of incubation times for all separation protocols
RoboSep™-S automatically executes all actions required to process up to four samples, with the flexibility to
simultaneously perform different types of separations.
2.1.1 Robotic Arm and Carousel
Robotic Arm
The robotic arm (Figure 3A) moves both vertically and rotationally. The stainless steel tip head at the end of
the arm is used to pick up pipette tips from the RoboSep™ Filter Tip Rack (Figure 3B). A custom, single-use
RoboSep™ Filter Tip Rack contains all the disposable pipette tips necessary to process a single sample.
Figure 3. (A) Robotic Arm and (B) RoboSep™ Filter Tip Rack. Note that the lid of the RoboSep™ Filter
Tip Rack needs to be removed prior to use.
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