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8.5 Liquid Handling Performance Issues
PROBLEM POTENTIAL CAUSE SOLUTION
No water ejected from the tip head
prior to run
Priming step missed or incomplete
Refill the hydraulic fluid bottle and
make sure the connection between
the bottle and tubing is secure by
disconnecting and reconnecting the
quick connect firmly
Leaking water from tip head onto
the tips' filter inserts
Compromised Teflon tube or leak in
the hydraulic system
Take a photo of the end of the tip
head and contact Technical
Support
Dripping of sample from pipette tips
onto carousel
Bad seal between the tip head and
disposable tip
Clean tip head and replace the tip
rack (use new tips)
A leak in the hydraulic system Clean tip head and replace the tip
rack (use new tips). If issue
persists, contact Technical Support.
Liquid transfer not taking place or
not accurate during a protocol run
Hydraulic fluid bottle has run out of
water
Refill hydraulic fluid bottle and run
Prime protocol (section 3.5)
3 - 5 times
Compromised Teflon tube or leak in
the hydraulic system
Take photo of the end of the tip
head and Contact Technical
Support
Hydraulic fluid sputtering out of
tip head/particulate matter present
in hydraulic fluid lines
Hydraulic fluid line contamination
Replace contents of hydraulic fluid
bottle with 70% isopropanol or
ethanol and prime > 4 times. Leave
alcohol in line for 1 hour. Replace
contents of hydraulic fluid bottle
with sterile deionized water and
prime > 5 times. If problem persists,
take a photo of the end of the tip
head and contact Technical
Support.
Backflow of sample/blood into
hydraulic fluid bottle
Faulty valves within pump
Take photo of hydraulic fluid bottle
and contact Technical Support
Reagents left in tips
This is a normal operation and is
required to meet the accuracy
requirements for low volume reagent
addition. The residual volume should
be 20 µL.
If the residual volume in the tips is
> 20 µL, take a photo of the tips in
question and contact Technical
Support
Residual diluted sample volume left
in tubes
Under most normal conditions,
RoboSep™-S leaves a residual
volume of 250 µL in tubes
If the volume of the diluted sample
is 250 µL, measured with a
micropipette, take a photo of the
tube in question and contact
Technical Support
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