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Figure 6. Carousel Gaskets. Gaskets at reagent vial and sample tube locations.
Homing the Robotic Arm and Carousel
RoboSep™-S tracks the position of the robotic arm and carousel relative to physical markers on the carousel
called homing flags. A home position is registered when an optical sensor detects a passing homing flag. The
carousel homing flag is visible as a black projection on the bottom plate of the carousel (Figure 7). Homing of
the carousel and robotic arm is performed automatically at startup, at the beginning of each cell isolation
protocol, and after the instrument has been paused during a run.
Figure 7. Carousel Homing Flag
To ensure accurate homing, it is essential that the arm and carousel are not obstructed while RoboSep™-S is
performing a cell separation protocol. To prevent such disruptions, RoboSep™-S operates with a transparent
lid over the arm and carousel. If the lid is opened during operation, the instrument will complete its current
action and pause. Once the lid is closed, RoboSep™-S will automatically execute a homing sequence and
resume the cell separation protocol where it left off.
2.1.2 Tip Stripping Arm
The disposable tips are returned to the same tip rack after each use; this eliminates the need for a separate
waste area on the instrument and facilitates easy cleanup after a run by allowing the entire tip rack to be
disposed of. RoboSep™-S uses the tip stripping arm to remove (i.e. strip) tips from the tip head once they
have been used. Figure 8 illustrates the automated 5 mL pipette tip stripping process.
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