PATIENT TESTING - 6
VerifyNow System User Manual Page 44 14340.J
• Greiner Bio-One Vacuette partial-fill blood collection tube (2 mL fill
volume) containing 3.2% sodium citrate (Greiner #454321) – only for use
with ReoPro (abciximab)
• 1 Label with patient ID (optional)
Blood Draw Procedure
Collection of blood sample should be performed with care to avoid hemolysis or
contamination by tissue factors.
1.
Locate a discard tube and the appropriate sample tube.
2.
Obtain samples from an extremity free of peripheral venous infusions according
to your institution’s phlebotomy procedures. The median antecubital and cephalic
veins are most commonly used.
3.
If drawing for a CBC at the same time, fill the CBC tube last to avoid
contamination by EDTA.
4.
Prepare the phlebotomy collection system using a 21 gauge or larger needle.
Butterfly (21 gauge or larger) is OK to use.
5.
Collect a discard sample of at least 2mL in a tube containing sodium citrate,
blood culture medium, no additive, or heparin (IIb/IIIa assay only).
6.
Fill the second tube (sample tube) to the black line. For the Greiner Vacuette
partial-fill tube (2 mL fill volume), 3.2% sodium citrate (#454321), the fill line is at
approximately ½ of the tube height (Figure 6-1). Do not under fill.
Figure 6-1 Sample Tube
7.
Gently invert the tube at least 5 times to ensure complete mixing of the contents.
Samples with evidence of clotting should not be used.
NOTE: Always ensure collection tubes are filled
to the indicated fill volume.
NOTE: At altitudes greater than 2500 feet above
sea level, blood collection tubes may not fill to
the specified volume, which results in an
incorrect ratio of blood to anticoagulant. Users at
these elevations should use tubes designed for
high altitudes or refer to their facility’s blood
collection protocols for instructions to properly fill
blood collection tubes.
Fill Volume
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