Agilent InfinityLab LC Series RID User Manual 109
8 Test Functions
Refractive Index Calibration
Preparing the Calibration
1
Preparation of the sucrose calibration solution.
a
To prepare 25 ml of the calibration solution 87.5 mg of the Sucrose
sample is required.
b
Add the weighed amount of sample into a suitable volumetric flask.
c
Dispense 10 ml of LC grade water into the flask and shake or stir to
dissolve.
d
Dilute the contents of the flask to volume with LC grade water.
Wait five minutes and shake again. The solution is now ready for use.
2
Preparing the pump.
a
Fill a suitable solvent bottle with LC grade water.
b
Connect this bottle to Channel A of the pump, A1 if a binary pump.
3
Flush the degasser and pump.
When
Recommended after exchange of the optical unit or RIM board.
Tools required Description
Laboratory balance
Parts required # p/n Description
1 DAB/Ph Eur/BP/JP/NF/USP Grade Sucrose
1 9301-1446 Syringe
1 9301-0407 Needle
1 5190-5111 Syringe filter, 0.45 µm, 100/pk
1 0100-1516 Fingertight fitting PEEK, 2/pk
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