58 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
4 Good Measurement Practices
General Considerations
Lens tissues for glasses or other uses often contain detergents or lubricants
which can affect your measurements. If possible, avoid cleaning the faces of
your cell between blank and sample measurements.
Handling Cells
Always install a cell so that it faces the same direction to minimize problems
with cell non-uniformity. For best results with microcells, leave your sample
cell clamped in position throughout the measurement sequence. Solutions
should be removed and replaced by pipette or use flow cells.
Figure 8 Spectrum taken with floating particles in the light path
If glass pasteur pipettes are used, make sure that the optical windows of the cell are
not touched or scratched by the pipette.
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