Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 69
Using your UV-Visible Spectroscopy System 5
Starting Your First Measurement Session
blank measurement performed. To start this measurement, click the Blank
button on your Instrument Panel or press the spectrophotometer’s Blank
button.
A blank measurement is a reference measurement combined with the
measurement of a baseline spectrum. A baseline spectrum gives you
additional hints on the absorbance of the cell windows and the solvent.
Areas with high noise indirectly indicate high absorbance.
7 The next measurement is your sample measurement. To get the most
precise results, use the same cell in the same orientation to the
measurement beam. Flush your cell about three times with your sample
solution and start the measurement by clicking the Instrument Panel’s
Sample button, or by pressing the spectrophotometer’s Sample button.
For high precision measurements, wait until the spectrophotometer and the lamps have
reached thermal equilibrium. The time required is a function of the environmental
conditions. Your spectrophotometer should be ready after 45 minutes.
For details on how to mount your cell, see “Inserting a Cell" on page 65.
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