Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 89
Using your UV-Visible Spectroscopy System 5
Finding the Caffeine Absorbance Maximum
4 Type 2 for the number of peaks to find and uncheck the Valley find option.
Set your data type to absorbance, and adjust your spectral display to a
wavelength range from 190 nm to 400 nm in the dialog’s Display spectrum
section. Click OK to set your parameters.
5 Fill your 1-cm path-length quartz cell with distilled water. Lift the lever at
the left side of your cell holder. Put the cell in the cell holder and make sure
the transparent windows face towards the front and the back of the
spectrophotometer. Push the lever down to secure your cell in the cell
holder.
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