Applied Biosystems 7500 Getting Started Guide

Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Creating a Relative Quantification (RQ) Plate Document
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 29
Notes
STANDARD
STANDARD
STANDARD
4
Sample Experiment
In the example RQ experiment, the samples for each of the three tissues (liver, kidney, and bladder) are loaded on three
separate plates. Consequently, three RQ Plate documents are created, one for each of the sample plates.
Because the experiment is singleplex, there is only one sample—either a target or endogenous control—in each well. Each
well is associated with a detector (indicated by the colored squares). Additionally, each well is assigned a detector task—T
(target) or E (endogenous control).
The figure below shows the example RQ Plate document after sample names, detectors, and detector tasks are assigned
for each well in the liver plate.
Sample name
Detector task and
color

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)