Applied Biosystems 7500 Getting Started Guide

Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Creating a Relative Quantification (RQ) Plate Document
28 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
STANDARD
STANDARD
STANDARD
8. Enter the sample names.
a. In the Well Inspector, click a well or click-
drag to select replicate wells.
b. Enter the sample name.
c. If necessary, change the setting for the
Passive Reference dye. (By default, the
ROX
™
dye is selected.)
d. Repeat steps a through c until you specify
sample names and passive reference dyes
for all the wells on the plate.
IMPORTANT! If your experiment does not
use all the wells on a plate, do not omit the
wells from use at this point. You can omit
unused wells after the run. For information
about omitting unused wells, refer to the
Online Help.
Note: You can change the sample setup
information (sample name, detector, task)
after a run is complete, if necessary.
e. Close the Well Inspector.
9. Verify the information on each well in the Setup
tab.
8a
8b
8e 8c

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)