DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Infrequent Maintenance
117
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
8. If the contamination persists, clean the wells with a 95% EtOH solution:
CHEMICAL HAZARD. Ethanol is a flammable liquid and
vapor. Exposure causes eye, skin, and respiratory tract irritation and may cause
central nervous system depression and liver damage. Read the MSDS, and follow
the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.
a. Perform steps 2 through 5, substituting a 95% EtOH solution in place of the
deionized water.
b. Repeat step 5 using deionized water to rinse the wells of the sample block(s).
c. Perform steps 6 and 7 to confirm that you have eliminated the contamination.
IMPORTANT! Use deionized water to rinse wells after they have been treated with
bleach or EtOH solution.
9. If the contamination persists, clean the wells with a 10% bleach solution:
CHEMICAL HAZARD. Sodium hypochlorite (bleach) is a
liquid disinfectant that can be corrosive to the skin and can cause skin
depigmentation. Read the MSDS, and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and powder-free gloves.
a. Perform steps 2 through 5, substituting a 10% bleach solution in place of the
deionized water.
b. Repeat step 5 using deionized water to rinse the wells of the sample block(s).
c. Perform steps 6 and 7 to confirm that you have eliminated the contamination.
IMPORTANT! Use deionized water to rinse wells after they have been treated with
bleach or EtOH solution.
10. If the contamination is still present, contact Applied Biosystems technical support.
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