Applied Biosystems StepOnePlus User Manual

153
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
DRAFT September 25, 2007 1:07 am, 4376782_IX.fm
Index
A
advanced setup 137
AIF. See assay information file.
allele 137
allelic discrimination plot 137
amplicon 137
amplification 137
plot 137
stage 138
amplification efficiency (EFF%) 137
analyze
background calibration 98
dye calibration 106
–109
RNase P experiment 43
–45, 68–70
spatial calibration 93
antivirus software 8
, 47, 71
APIPA. See Automatic Private IP Addressing.
Applied Biosystems
contacting x
customer feedback on documentation ix
Information Development department ix
Technical Support x
temperature verification service 89
Applied Biosystems StepOne and StepOnePlus Real-
Time PCR System. See instrument or system.
archiving files 113
assay 138
ID 138
mix 138
assay information file (AIF) 138
AutoDelta 138
automatic baseline 138
automatic C
T
138
Automatic Private IP Addressing compatibility 78
B
back up experiments 113
background calibration 94
–99
analyze the data 98
guidelines 94
perform 96
prepare 95
–96
purpose 94
background component, about 94
background plate
about 94
create 99
number of reuses 95
backup storage devices 7
baseline-corrected normalized reporter (∆Rn) 139
baseline 138
biohazardous waste, handling xvii
bleach solution, usage 115
blocked IPC 139
bold text, use of viii
C
calibration
background 94
–99
dye (custom) 110
–112
dye (system) 100
–108
schedule 88
spatial 90
–93
calibrator 139
CAUTION, description xi
chemical
safety xv
waste safety xvii
chemistry 139
chemistry installation kit 18
clearance requirement, instrument 15
colocated layout 9
, 139
connect system components 35
–37
connect to a network 74
example network layout 75
install computer 27
–34
installation 26
–47
installation workflow 26
perform RNase P experiment 38
–46
when to install 26
comparative C
T
(∆∆C
T
) method 139
computer
connect to instrument 35
default user name and password 30
, 53
monitor instrument 82
name, define 33
, 56
placement 28
, 52
prepare operating system 31
–33, 54–56
remote monitoring set up 80
requirements 6
site preparation 28
, 52

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English