Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
96
Notes
7. Confirm that the liquid is at the bottom of each well of the background plate. If not,
centrifuge the background plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the background plate to become dirty.
Fluids and other contaminants that adhere to the bottom of the background plate can
contaminate the sample block(s) and cause an abnormally high background signal.
8. Load the background plate into the instrument:
a. Open the instrument drawer.
b. Place the background plate in the sample block(s) so that the A1 position is at
the back-left corner.
c. Close the instrument drawer carefully.
Perform the
Calibration
1. In the main screen of the StepOne
™
software, select Instrument
Instrument Maintenance Manager.
2. In the navigation pane of the Instrument Maintenance Manager, click Background.
3. Click Start Calibration.
4. In the Setup screen of the Background Calibration dialog box, select The
background plate is loaded into the instrument, then click Next.
5. In the Run screen, click START RUN , then wait for the instrument to complete
the background calibration.
Note: If the StepOne
™
software displays messages during the run, troubleshoot the
errors as described in “Background Calibration Failure” on page 132 .
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force, or
• Not centrifuged for enough time

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English