EasyManuals Logo

Applied Biosystems StepOnePlus User Manual

Applied Biosystems StepOnePlus
184 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #84 background imageLoading...
Page #84 background image
DRAFT
September 25, 2007 1:07 am, 4376782_Standalone_Installation.fm
Chapter 4 Install the Standalone Layout
Perform the RNase P Experiment
Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
62
Notes
Set Up the Experiment
Prepare the TaqMan
®
RNase P Fast Instrument Verification Plate for the run.
Prepare the
RNase P Plate
IMPORTANT! Do not use an RNase P plate for another Applied Biosystems instrument
to verify the performance of the StepOne
system. RNase P plates for other instruments
contain the TAMRA
dye, which is not supported by the StepOne
system.
IMPORTANT! Wear powder-free gloves when you handle the
RNase P plate
.
1. Get the TaqMan
®
RNase P Fast Instrument Verification Plate from the freezer, then
allow the reaction plate to warm to room temperature (for approximately 5 min).
2. Remove the
RNase P
plate from its packaging.
3. Vortex the RNase P plate for 5 sec.
4. Centrifuge the RNase P plate for 2 min at less than1500 rpm.
IMPORTANT! The RNase P plate must be well mixed and centrifuged.
5. Confirm that the liquid is at the bottom of each well of the RNase P plate. If not,
centrifuge the plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the RNase P plate to become dirty. Fluids
and other contaminants that adhere to the bottom of the RNase P plate can
contaminate the sample block(s) and cause an abnormally high background signal.
Correct Incorrect
Liquid is at the
bottom of the well.
Not centrifuged with enough force,
or
Not centrifuged for enough time

Table of Contents

Questions and Answers:

Question and Answer IconNeed help?

Do you have a question about the Applied Biosystems StepOnePlus and is the answer not in the manual?

Applied Biosystems StepOnePlus Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOnePlus
CategoryLaboratory Equipment
LanguageEnglish

Related product manuals