DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
98
Notes
Analyze the
Calibration Data
1. In the Analysis screen of the Background Calibration dialog box, confirm the status
of the calibration:
• Passed – The instrument passed the calibration. Go to step 2.
• Failed – The instrument failed the calibration. Troubleshoot the error as
described in
“Background Calibration Failure” on page 132
.
2. Select all wells of the plate layout.
3. Inspect the raw data for irregular spectral peaks.
If one or more wells produce raw spectra that diverge significantly from that of the
neighboring wells, the background plate or the sample block(s) could contain a
fluorescent contaminant. If you suspect contamination, troubleshoot the irregular
signal as explained in
“Background Calibration Failure” on page 132
.
4. If all spectra are acceptable, click Finish.
5. When prompted to save the background calibration, click Ye s .
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