Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_IX.fm
Index
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
154
Computer Port
about 76
connect the computer and instrument 29
connect, instrument to computer 35
contamination, removal 115
–117
conventions
bold text viii
for describing menu commands viii
IMPORTANTS! viii
in this guide viii
italic text viii
Notes viii
safety xi
user attention words viii
create
background plate 99
custom dye plate 110
–112
C
T
. See threshold cycle.
custom dye
about 139
calibration 110
–112
plate, how to create 110
–112
customer feedback, on Applied Biosystems
documents ix
cycle threshold 139
cycling stage 140
D
DANGER, description xi
data
about collection 3
, 140
backup/storage devices 7
date and time settings
define for the instrument 21
define for the software 32
, 55
delta Rn (∆Rn) 140
derivative reporter (- Rn′ ) 140
Design Wizard 140
diluent 140
diluted sample concentration 140
dilution factor 140
dimensions
computer 28
, 52
instrument 15
disable 'Local Area Connection' messages 37
disk space, checking 113
dissociation curve 140
documentation
obtain x
related ix
shipped with the system 19
dye calibration 100
–108
analyzing data 106
analyzing the data 106
–109
perform 105
–106
prepare 103
–104
purpose 100
spectra, evaluating 102
dye plate
about 100
create 110
custom dye 100
dyes
custom 101
, 139
system 4
, 101, 149
E
EFF%. See amplification efficiency.
electrical protective devices 7
electrical safety xviii
electromagnetic compatibility standards.
See EMC standards
EMC standards xx
encryption software 8
, 47, 71
endogenous control 140
endpoint read 140
ergonomics, safety xix
experiment 141
download from instrument 84
name 141
RNase P. See RNase P experiment.
send to instrument 84
type 141
experiment workflow
colocated layout 9
standalone layout 10
F
FAM
™
system dye 4, 101
spectral profile 108
, 109
file compression software 8
, 47, 71
firewall software 8
, 47, 71
forward primer 141
G
guidelines
background calibration 94
bleach solution, usage 115
chemical safety xv
chemical waste disposal xvii
chemical waste safety xvii
lifting and moving 16
, 28, 52
networking 77
remote monitoring 82

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English