Applied Biosystems StepOnePlus - Page 125

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
103
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
Prepare for the
Calibration
IMPORTANT! Wear powder-free gloves when you handle the calibration plates.
1. If you have not already done so, use the StepOne system cable to connect the:
• Yellow Ethernet port ( ) of the instrument to the
• Ethernet port ( ) of the computer running the StepOne
™
software
IMPORTANT! Do not connect the StepOne system cable to the blue LAN Port ( ).
2. Get calibration plate 1 from the spectral calibration kit in the freezer.
3. Allow calibration plate 1 to warm to room temperature (~5 min).
IMPORTANT! Do not remove calibration plate 1 from its packaging until you are
ready to run it. The fluorescent dye in the wells of each calibration plate is
photosensitive. Prolonged exposure to light can diminish the fluorescence signal
strength of the dyes.
4. Remove calibration plate 1 from its packaging.
5. Vortex calibration plate 1 for 5 sec.
6. Centrifuge calibration plate 1 for 2 min at less than1500 rpm.
IMPORTANT! The calibration plate must be well mixed and centrifuged.
Ethernet